File: Protein Purification Slideshare 66821 | Gel Filtration Chromatography
objectives 1 the objective of this experiment is for students to learn the principles of gel filtration chromatography by separating of two substance according to the molecular weights 2 a ...
Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago
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...Objectives the objective of this experiment is for students to learn principles gel filtration chromatography by separating two substance according molecular weights a practical experience on in laboratory importance and procedures purification protein isolation series processes intended isolate one or few proteins from complex mixture usually cells tissues whole organisms cell debris other are removed centrifugation could you will have be salting out that different tissue homogenization charges sizes properties techniques used separate molecules size shape pass through medium packed column move bed porous beads diffusing into greater lesser degrees mobile phase stationary nacl buffers matrix lab hydrated polymer made consisting containing pores defined range...