Blotting Slideshare 67024 | Western Blotting Protocol

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File: Blotting Slideshare 67024 | Western Blotting Protocol

western blotting protocol i prepare protein samples ii sds page iii membrane transfer iv preliminary staining v cutting membrane vi blocking vii primary antibody viii secondary antibody ix chemiluminescent treatment ...

Filetype Power Point PPTX | Posted on 28 Aug 2022 | 3 years ago

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...Western blotting protocol i prepare protein samples ii sds page iii membrane transfer iv preliminary staining v cutting vi blocking vii primary antibody viii secondary ix chemiluminescent treatment x imaging make a polyacrylamide gel load into run determine percent see chart to best clean cassette and ensure sealing corresponding resolving stacking the latter without temed add up notch wait minutes or until solidified on top of remove from place electrophoresis apparatus its aligns with short plate faces inwards spacer outwards rear plastic piece fits snugly tighten clamps both sides internal chamber are sealed in container fill middle running buffer spill over larger lower electrode is covered pipette loading dye use contains beta mercaptoethanol wells rinsing tip between each sample by pipetting through ladder penultimate well only first last all should be equivolumetric set machine for min periodically check if still er means something wrong likely loose electrical connection stop i...

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Posted on: 28 Aug 2022 | 3 years ago
Tag: download pptx, western, blotting, protocol,
Filetype: PPTX
File size: 0.13 MB
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