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picture1_Gene Sequencing Slideshare 68137 | Lesson4 4 Genomeassembly


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File: Gene Sequencing Slideshare 68137 | Lesson4 4 Genomeassembly
Assembling a gene sequence Align sequencing reads to generate a series of overlapping sequences that cover the gene. Sequencing both strands is more accurate. 08/28/2022 Hardison, R (1983) J. Biol ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago
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...Assembling a gene sequence align sequencing reads to generate series of overlapping sequences that cover the both strands is more accurate hardison r j biol chem multiple sequencher codes corp stephan schuster contig assembly libraries with different insert sizes gap plasmid library kb bac dealing gb not mb genomes vary over orders magnitude bacterial about yeast saccharomyces cerevisiae fly drosophila melanogaster plant arabidopsis thaliana thale cress fish danio rerio zebrafish bird gallus chicken human homo sapiens reference organize information use pre existing maps if available genetic radiation hybrid physical clone current focus on paired end substantial genome coverage x drive de novo linkage relative locations specific dna markers along chromosome phenotype tagged sites stss always examine polymorphic breaks during meiotic recombination give an opportunity separate limited need be random induced in chromosomes pieces hamster cells associations above can meaningful fluorescent ...

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