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picture1_Blotting Slideshare 67182 | 51 6th Sem Western Blotting Debabrata Paria


picture2_Blotting Slideshare 67182 | 51 6th Sem Western Blotting Debabrata Paria picture3_Blotting Slideshare 67182 | 51 6th Sem Western Blotting Debabrata Paria

 137x       Filetype PPTX       File size 0.86 MB       Source: www.bajkulcollegeonlinestudy.in


File: Blotting Slideshare 67182 | 51 6th Sem Western Blotting Debabrata Paria
Western blotting/Immunoblotting Technique for detecting specific proteins separated by electrophoresis by use of labeled antibodies. Allows to identify a particular protein of interest among many proteins in a sample. Introduced ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago

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...Western blotting immunoblotting technique for detecting specific proteins separated by electrophoresis use of labeled antibodies allows to identify a particular protein interest among many in sample introduced towbin et al and is now routine analysis the specificity antibody antigen interaction enables single be identified midst complex mixture flow chart electrophoresing assembling blot sandwich transferring from gel nitrocellulose paper staining transferred blocking nonspecific sites on probing electroblotted with primary washing away nonspecifically bound horseradish peroxidase anti ig conjugate formation diaminobenzidine dab precipitate photographing immunoblot sds polyacrylamide page sodium dodecyl sulfate stands thin separates molecules basis size negatively charged are loaded into wells that submerged buffer electrical current applied causing move toward positive electrode anode usually marked red smaller faster through than larger thus will found at bottom top...
								

							

						
	
												
				
					
					
					
			
					
				
				
		
	

	
		





	

	
				

						

						
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										Category:  Uncategorized / Uncategorized 
										
Posted on: 28 Aug 2022 | 2 years ago
										
										
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Filetype: PPTX
										
File size: 0.86 MB
										
Views: 137x
										
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												Wan learn