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...Western blotting also known as immunoblotting or protein is a core technique in cell and molecular biology most basic terms it used to detect the presence of specific complex mixture extracted from cells procedure relies upon three key elements accomplish this task separation mixtures by size using gel electrophoresis efficient transfer separated proteins solid support detection target appropriately matched antibodies once detected will be visualized band on membrane x ray film an imaging system accomplished rapidly simple equipment inexpensive reagents overview shown diagram below extremely powerful despite its overall simplicity because provides additional information not readily gathered other immunological laboratory techniques since are during stage then specifically directed antibody essentially confirms identity blots wide use across broad range scientific clinical disciplines their ability clearly show both through binding makes them well suited for evaluating levels expression...