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...Introduction all are physical separation processes involved in upstream vaccine purification centrifugatio filtration dialysis n function isolating dna rna proteins etc mechanism density reliant process using centrifugal forces centrifugation equation rotations per minute rpm relative distance force cm parameters low speed high ranges maximum rcf removal of cell debris clarification step production particle size pressure gradient types tubular tangential flow hollow fiber or plate and frame depth membrane general change across filter volume area concentration solid filtrate initial mixture viscosity over time other terms resistance r alpha...