Introduction • particles or cells in a liquid suspension, will settle at the bottom of tube due to gravity (1x g) during long period to time. • However, the length of time required for such separations is impractical. • Other particles which are very small in size, will not separate at all • So, high centrifugal force though method called centrifugation must be used in these cases. Simply, Centrifugation >>>>> Increasing the effect of gravity Sedimentation • Is the motion of molecules in solutions or, particles in suspensions, in response to ...

 

Introduction All are physical separation processes involved in upstream vaccine purification. [1] [2] [3] Centrifugatio Filtration Dialysis n [5 Centrifugation ] Function: Isolating DNA, RNA, proteins, etc. Mechanism: Density reliant process; separation using centrifugal forces [4 ] Centrifugation: Equation 6 Rotations per Minute (rpm) Relative Centrifugal Distance Force (cm) Parameters Low Speed High Speed Speed Ranges (rpm) 2000 - 6000 18,000 - 22,000 Maximum RCF 8,000 60,000 Filtration Function: Removal of cell debris (“clarification” step of vaccine production) Mechanism: Particle size, pressure gradient Types: Tubular Pressure Filtration Tangential Flow Filtration (Hollow Fiber or Plate and ...