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picture1_Southern Blotting Slideshare 66976 | 1638209763btg Blotting


picture2_Southern Blotting Slideshare 66976 | 1638209763btg Blotting picture3_Southern Blotting Slideshare 66976 | 1638209763btg Blotting

 129x       Filetype PPTX       File size 2.10 MB       Source: portal.abuad.edu.ng


File: Southern Blotting Slideshare 66976 | 1638209763btg Blotting
Blotting • All techniques use electrophoresis to separate. • All are based on hybridization technique • Difference in techniques lies in the target • Four applications –Western –Southern ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago
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...Blotting all techniques use electrophoresis to separate are based on hybridization technique difference in lies the target four applications western southern northern southwestern binding between ss labeled probe a complementary nucleotide sequence dna degree of depends method labeling radioacitve or non radioactive system e g biotin avidin probes material detect for nucleotides region gene methods p sensitive relatively cheap expensive hazardous you should follow waste disposal regulations blots named after british biochemist edwin em detection specific sequences among fragments separated by gel j mol biol nov identifying which transferred membrane and identified with suitable detects restriction following enzyme digest genomic rflps rt pcr can be used screen cdna libraries referred as blot is one most commonly molecular using process that transfer into nitrocellulose nylon...
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