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...Setting up a tissue culture lab any laboratory in which techniques are performed regardless of the specific purpose must contain number basic facilities these usually include following general washing area media preparation sterilization and storage an aseptic transfer environmentally controlled incubators or rooms observation data collection nutrient type composition very strongly govern growth morphogenesis plant tissues choice medium largely depends upon species to be cultured for e g some sensitive high salts have different requirements pgrs show better response on solid while others prefer liquid therefore development formulations is result systematic trial experimentation considering particular system white s one earliest originally formulated root murashige skoog ms most suitable commonly used regeneration from callus this salt due its content potassium nitrogen b works well protoplast it has lesser amounts nitrate particularly ammonium than nitsch developed anther contains conc...