chromatography chromatography is usually introduced as a technique for separating and or identifying the components in a mixture the basic principle is that components in a mixture have different tendencies ...
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...Chromatography is usually introduced as a technique for separating and or identifying the components in mixture basic principle that have different tendencies to adsorb onto surface dissolve solvent it powerful method industry where used on large scale separate purify intermediates products various syntheses classification of theory there are several types currently use iepaper thin layer tlc gas gc liquid lc high performance hplc ion exchange gel permeation filtration principles all chromatographic methods require one static part stationary phase moving mobile techniques rely following phenomena adsorption partition molecular exclusion was developed first has solid gaseous plant pigments were separated at turn th century by using calcium carbonate hydrocarbon solutes travelled distances through carried along each solute its own equilibrium between solubility least soluble best adsorbed ones travel more slowly result separation into bands containing column silica alumina an example fig...