section 1 general notices for microbiological assays water reagents and instruments to be used in microbiological assays shall be sterilized before use as occasion demands 1 plate method a reagent ...
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...Section general notices for microbiological assays water reagents and instruments to be used in shall sterilized before use as occasion demands plate method a reagent preparation buffer solutions prepare directed below sterilize an autoclave at c minutes if ph adjustment is needed phosphoric acid mol l or potassium hydroxide solution i no weigh g of dihydrogen phosphate dissolve ml adjust the add make ii disodium hydrogenphosphate above amounts iii iv about v dipotassium hydrogen vi sodium chloride mentioned vii viii lactic ix carbonate x acetic boric xi dihydroxybenzene disulfonic salt xii standard individual monograph stock using working standards currently specified conventionally appendix ordinance concerning ingredient specificationsministerial specifications feed additives issued by ministry agriculture forestry environment relative humidity lower store hermetic container culture media with compositions values listed following table hydrochloric per medium f peptone pancreatic di...