USING AN AUTOCLAVE FOR STERILZATION These notes are for reference only and are not intended to replace training by your lab supervisor. Ensure you familiarize yourself with your lab protocols and the appropriate safety data sheets (SDS). TRAINING IS IMPORTANT TO HELP YOU UNDERSTAND; what the potential risks are to you or others how to ensure proper sterilization of your run how to use and care for the equipment BASIC PRINCIPLES OF AN AUTOCLAVE: steam is produced from the boiler or steam generator inside the autoclave steam enters the chamber of the autoclave and contacts cooler surfaces the steam condenses ...
GN 4 - Guidance Notes on the use of bench top steam sterilizers This Authority's advice in relation to the use of bench top steam sterilizers is given as follows: Bench Top Steam Sterilizer – (autoclave) The most reliable method of sterilising equipment is moist heat using steam under pressure i.e. autoclave. Autoclaves operate at moist heat temperatures of between 121ºC and 134ºC. It is important that the correct type of autoclave is purchased for the type of sterilisation required. The type of equipment and quantity of equipment placed in it will also dictate the autoclave ...
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