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...Using an autoclave for sterilzation these notes are reference only and not intended to replace training by your lab supervisor ensure you familiarize yourself with protocols the appropriate safety data sheets sds is important help understand what potential risks or others how proper sterilization of run use care equipment basic principles steam produced from boiler generator inside enters chamber contacts cooler surfaces condenses causes a decrease in volume creating negative pressure which draws more condensation occurs as long there temperature differential penetrates contents moist heat kills organisms coagulating proteins o typical temp c some autoclaves operate at inactivate prions e g chronic wasting disease most require cycles minimum minutes achieve primary when high may cause burns scalds shelving hot opening door autoclaved liquids could boil over explode if vented container tightened lid would pressurize improper lead biological hazards risk personnel environment personal pr...