Using molecular marker technology in studies on plant genetic diversity DNA-based technologies Restriction fragment length polymorphisms Copyright: IPGRI and Cornell University, 2003 RFLPs 1 Contents ! RFLP technology • Isolating DNA • Restriction digestion and gel electrophoresis • DNA transfer by Southern blotting • DNA hybridisation • Equipment ! RFLP technology in pictures ! Interpreting RFLP bands ! Advantages and disadvantages of RFLPs ! Applications • Maize • Wheat &bull ...
DNA-FINGERPRINTING MENGGUNAKAN PENANDA PCR-RFLP UNTUK IDENTIFIKASI DAN AUTENTIFIKASI Hylarana chalconota (SHLEGEL, 1837) KOMPLEKS THESIS RYSKI DARMA BUSTA 1521652006 DIBAWAH BIMBINGAN DR. DJONG HON TJONG, M. Si DR. DEWI IMELDA ROESMA, M. Si PROGRAM PASCASARJANA UNIVERSITAS ANDALAS PADANG, 2018 DNA-FINGERPRINTING MENGGUNAKAN PCR RFLP UNTUK IDENTIFIKASI DAN AUTENTIFIKASI Hylarana chalconta (SHLEGEL, 1837) KOMPLEKS Oleh : Ryski Darma Busta (1521652006) (Dibawah bimbingan : Dr. Djong Hon Tjong dan ...
Hemera Zoa I Majalah Zlmu Kehewanan Zndonesia ( Indonesian Jwml of Veterinary science & ~eiicine Restriction Fragment Length Polymorphism (RFLP) Gen Sitokrom b dari Delapan Species Burung The Restriction Fragment Length Polymorphism (RFLP) of Mitochondria1 Cytochrome b Gene of Eight Birds Species D. A. Wibowo, W.E. Prasetyaningtyas dan I. Djuwita* dan Farmakologi, Fakultas Kedokteran Hewan Departemen Anatomi, Fisiologi Institut Pertanian Bogor, Jalan Agatis ...
The Plant Cell, Vol. zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA1, 699-705, July 1989 O 1989 American Society of Plant Physiologists Restriction Fragment Length Polymorphism Linkage Map of zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBAArabidopsis thaliana Hong-Gil Nam,' JerOme Giraudat, Bart den Boer,' Francis Moonan, William D.B. Loos, Brian M. Hauge, and Howard M. Goodman3 Department of Genetics, Harvard Medical School and Department of Molecular Biology, Massachusetts General Hospital, Boston. Massachusetts 021 14 We ...
June 2009 KUWAIT MEDICAL JOURNAL 117 Original Article Heat Treatment of Bacteria: A Simple Method of DNA Extraction for Molecular Techniques 1 1 2 3 Ali A Dashti , Mehrez M Jadaon , Abdulsamad M Abdulsamad , Hussein M Dashti 1 Department of Medical Laboratory Sciences, Faculty of Allied Health Sciences, Health Science Center, Kuwait University, Kuwait 2 Department of Surgery, Amiri Hospital, Kuwait 3 Department of Surgery ...
DNA Variation VNTR, STR, RFLP, RAPD, AFLP, VNTR and DNA Sequencing Fatchiyah Objectives • Briefly describe VNTR, STR and RFLP • Describe the principle, including the target, for: Southern, Western, Northern and Southwestern blots. • Compare and contrast the 4 blotting methods. • Given a target state the best method for detection. • State 2 clinical applications for detecting VNTRs, STRs and RFLPs. • Given ...
® HiPer Restriction Fragment Length Polymorphism (RFLP) Teaching Kit Product Code: HTBM026 Number of experiments that can be performed: 5/25 Duration of Experiment Protocol:3.5 hours Agarose Gel Electrophoresis:1 hour Storage Instructions: ¾ The kit is stable for 12 months from the date of receipt ¾ Store DNA samples, 1 kb DNA ladder, Restriction Enzymes, 6X Dye o and Buffers at-20 C ¾ Other reagents ...
Journal of J Appl Biotechnol Rep. 2019 Dec;6(4):137-144 Applied Biotechnology doi 10.29252/JABR.06.04.02 Reports Review Article Exploring the Potential and Limitations of PCR-RFLP and PCR-SSCP for SNP Detection: A Review 1 2* Hayder O. Hashim , Mohammed Baqur S. Al-Shuhaib 1Department of Clinical Laboratory Sciences, College of Pharmacy, University of Babylon, Babil 51001, Iraq 2Department of Animal Production, College of Agriculture, Al-Qasim ...
TEKNOLOGI DNA REKOMBINAN (RECOMBINANT DNA TECHNOLOGY) IDA SRI ISWARI RSUP SANGLAH, DENPASAR-BALI KEMENENTERIAN KESEHATAN REPUBLIK INDONESIA DAFTAR ISI DAFTAR ISI i BAB I PENDAHULUAN 1 BAB II TEKNOLOGI MOLEKULER DNA 3 2.1 Polymerase Chain Reaction (PCR) 3 2.1.1 Reverse Transcription-PCR (RT-PCR) 7 2.1.2 PCR-Single-Strand konformasi Polymorphism (PCR-SSCP) 8 2.1.3 The Ligase Chain Reaction (LCR) 10 2.2 Kloning ...
DNA extraction methods Dr.Lalani Yatawara Department of MLS, FAHS Dr.L.Yatawara How Can We Recover DNA From a Variety of Sources of Biological Evidence? Blood Cigarette Butts Semen Envelope & Saliva Stamps Urine Fingernail Hair (w/Root & Shaft) Clippings Teeth Chewing Gum Bone Bite Marks Tissue Feces Dr.L.Yatawara What are the essential components of a DNA extraction Procedure? 1 ...
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