Pour Plate Method: Best Practices Best Practices for Pour Plate Method —————————— • According to a consensus of views, it is recommended to keep the molten agar in the water bath for no more than 3 to 4 hours; do not pour it until it has cooled to ...
LeveL 3 SoiL microbioLogy Lab.3 iSoLation and numeration of SoiL microorganiSm Soil is a suitable environment for a diverse of microbial community consists of bacteria, actinomycetes, molds, yeast, algae, and protozoa. Due to differences in nutritional requirements for different type of microorganism in the soil its essential to use different types of culture media for isolation and numeration. 1- Nutrient agar: for numeration of ...
1 Microbiological Methods I-A- MESOPHILIC AEROBIC BACTERIA (STANDARD PLATE COUNT OR TOTAL PLATE COUNT) PRINCIPLE Viable bacteria are quantitated by the fractional gram pour plate technique. SCOPE The method is applicable to starches, syrups and sugars and most coproducts of the corn wet milling industry. MEDIA AND REAGENTS 1. Plate Count Agar (PCA). Prepare according to the manufacturer's directions. 2. Butterfield's Phosphate Diluent ...
1 Microbiological Methods VIII-B- MESOPHILIC ANAEROBIC BACTERIA (POUR PLATE METHOD) PRINCIPLE Viable anaerobic bacteria are quantitated by the fractional gram pour plate technique under an anaerobic atmosphere. Caution must be exercised when applying the method since isolates may be pathogenic. SCOPE The method is applicable to starches, syrups, sugars and most co-products of the corn wet milling industry. SPECIAL APPARATUS Anaerobic jars, BBL GasPak or ...
Microbiology Laboratory (BIOL 3702L) Page 1 of 8 POUR PLATE DETERMINATION OF BACTERIA NUMBERS Principle and Purpose Some microbiological investigations require the determination of the number of microbes present in a culture, solution, etc. There are several means of making this quantitative measurement. In this exercise, students will use two commons methods: the standard plate count and spectrophotometric analysis. The former is based upon the ...
1 Microbiological Methods IV-A- COLIFORM GROUP OF BACTERIA (STANDARD PLATE COUNT METHOD) PRINCIPLE Coliform bacteria are quantitated by the fractional gram pour plate technique (Note 1). SCOPE The method is applicable to starches, syrups and sugars of the corn wet milling industry. SPECIAL APPARATUS Test tubes containing gas collector tubes (Durham Tubes) MEDIA AND REAGENTS 1. Brilliant green lactose bile broth (BGLB), 2% (Note 2) ...
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