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...Microbiological assay of antibiotics vitamins amino acids labmonk com introduction is a technique in which the potency or concentration compound assessed by determining its effect on micro organisms principles are discussed roberts and boyce required for number both british pharmacopoeia bp united states usp bioassay compares reference standard an unknown sample two preparations being measured simultaneously lot preliminary work necessary assays if only small samples expected irregularly method inefficient methods normally used agar diffusion tube they have several common features assayed must influence growth test organism varying response be produced addition quantities material medium contain excess all compounds exception to this should totally absent from basic assumption made that promoting inhibiting present principle based upon comparison inhibition examined with known concentrations preparation antibiotic having activity general usually employed cylinder plate cup turbidimetri...