exam mol 3008 may 2009 subject 1 15p alp alkaline phosphatase calibrators were analyzed manually in microtiter plates to find the linearity range by following this protocol reaction solutions 50 ...
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...Exam mol may subject p alp alkaline phosphatase calibrators were analyzed manually in microtiter plates to find the linearity range by following this protocol reaction solutions l of calibrator control sample is added each plate well duplicates buffer substrate reagent all wells start timer when adding first cover with parafilm and put into a c incubator incubate for minutes m naoh same order as blanking add these new o no incubation read absorbance reader at nm subtract blank from calibration factor curve calculate results table shows concentrations average solution u linear analysis draw diagram explain mm paper not necessary what would you do if some your samples measured above method change s improve sensitivity describe technique called western blot or immuno blotting suggest strategies how ensure that signal get represents correct protein b like visualize localization two different proteins antigen simultaneously staining considerations have previous kind controls could specifici...