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...Aim isolate the fungi from given soil sample wet mount and identify principle isolation identification of is an example in which a mycological ecosystem particular habitat with its interacting associated community both abiotic biotic can be studied it usually too difficult to separate by directly picking them place under microscope for observation therefore various techniques have been devised stimulating growth consequently easing fungal subsequently examination used include serial dilution agar plate warcup syringe inoculation immersion tube method screened plates profile hyphal washing partial presterlization sieving floatation baiting etc single cannot count all different types present so complete spectrum processed variety methods are two most widely rapid routine almost spores spore bearing structures tested out on slide drop mounting fluid lactophenol cotton blue cover glass placed over preparation then ready microscopic requirements potato dextrose media sterile petri test tube...