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...Bacterial culture media one of the most important reasons for culturing bacteria in vitro is its utility diagnosing infectious diseases isolating a bacterium from sites body normally known to be sterile an indication role disease process also initial step studying morphology and identification have cultured order obtain antigens developing serological assays or vaccines certain genetic studies manipulations cells need that provide reliable way estimating their numbers viable count on solid another convenient separating mixtures history louis pasteur used simple broths made up urine meat extracts robert koch realized importance potato pieces grow it was suggestion fannie eilshemius wife walther hesse who assistant agar solidify before use attempts were gelatin as solidifying agent had some inherent problems existed liquid at normal incubating temperatures oc digested by composition infecting humans commensals pathogens are chemoorganoheterotrophs when very similar environmental nutritio...