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picture1_Hybridoma Technology Pdf 89790 | Article 017


picture2_Hybridoma Technology Pdf 89790 | Article 017 picture3_Hybridoma Technology Pdf 89790 | Article 017

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File: Hybridoma Technology Pdf 89790 | Article 017
Volume 1, Issue 2, March – April 2010; Article 017 ISSN 0976 – 044X HYBRIDOMA TECHNOLOGY FOR PRODUCTION OF MONOCLONAL ANTIBODIES Shivanand Pandey Smt. R. B. P. M. Pharmacy College ...

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...Volume issue march april article issn x hybridoma technology for production of monoclonal antibodies shivanand pandey smt r b p m pharmacy college atkot rajkot gujarat india email dot gmail com abstract hybridomas are cells that have been engineered to produce a desired antibody in large amounts removed from the spleen an animal has challenged with relevant antigen these then fused myeloma tumor can grow indefinitely culture is cell cancer this fusion performed by making membranes more permeable hybrid called being will multiply rapidly and they be selected subsequently cloned limiting dilution supplemental media containing interleukin such as briclone essential step anti bodies was first invented cesar milstein georges j f kohler niels kaj jerne selection occurs via culturing newly primary selective specifically concentration hat roughly days after using it often desirable use ht cloning identification positive clone limited while some may believe il not necessary add expensive supple...
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