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...Harris hematoxylin eosin stain histology staining description is used for specimen and this regressive appears darkest most intense in appearance use if the gill x not providing a dark enough nuclear alternatively acidified as progressive method fewer steps same good quality protocol h e paraffin slides deparaffinize xylene then rehydrate through graded alcohols to water frozen fix ethanol or other fixative according lab preference rinse solution minutes two changes of di minute each note could also be done using running tap but we recommend avoid varying ph found acid alcohol seconds some prefer weaker which while others higher percent will extract dye little fast there much difference from here bluing reagent until tissue turns blue y alcoholic intensified phloxine an increased definition cytoplasmic components over select alternative dehydrate dips clear substitute coverslip acrylic mounting medium examined under microscope ordering information cat sizes deionized gallon hg free oz ...