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...Hematoxylin and eosin stain h e technique formalin fixed paraffin tissue sections reagents mayer s source medical catalog acid alcohol ethanol ml hydrochloric x pbs ph alcoholic y cas g distilled water phloxine b working solution acetic glacial micron procedure deparaffinize rehydrate slides to in mayers for minute wash with changes of tap or until blue stops coming off nuclei counterstain do not rinse dehydrate through etoh each clear xylene change mount coverslip after go directly into dehydration results cytoplasm pink erythrocytes bright red all other eosinophilic structures orange fresh frozen section take immediately from freezer place cold fixative neutral buffered pfa minutes times remove oct embedding compound a gentle stream seconds dip is washed...