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picture1_Gene Transfer Methods Pdf 89581 | Direct Gene Transfer


picture2_Gene Transfer Methods Pdf 89581 | Direct Gene Transfer picture3_Gene Transfer Methods Pdf 89581 | Direct Gene Transfer

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File: Gene Transfer Methods Pdf 89581 | Direct Gene Transfer
B) Direct Gene Transfer: The term direct transfer of gene is used when the foreign DNA is directly introduced into the plant genome. Direct DNA transfer methods rely on the ...

icon picture PDF Filetype PDF | Posted on 15 Sep 2022 | 2 years ago

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...B direct gene transfer the term of is used when foreign dna directly introduced into plant genome methods rely on delivery naked cells can be broadly divided two categories a physical electroporation microinjection particle bombardment chemical poly ethylene glycol peg mediated diethyl amino ethyl deae dextran calcium phosphate precipitation this method involves suspension protoplasts in suitable ionic solution containing linearized recombinant plasmid mixture then exposed to low voltage long pulses or high short for desired number cycles electrical are thought induce transient pores plasma lemma through which molecules incorporated treated cultured obtain cell colonies and plants called i e introduction by making minute membrane involving mechanical insertion desirable target may one identified from intact callus embryos meristems etc cellular organelles manipulation chromosomes technique micropipette pm tip cytoplasm nucleus protoplast while done recipient kept immobilized agarose em...
								

							

						
	
												
				
					
					
					
			
					
				
				
		
	

	
		





	

	
				

						

						
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										Category:  Uncategorized / Uncategorized 
										
Posted on: 15 Sep 2022 | 2 years ago
										
										
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Filetype: PDF
										
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												Wan learn