Thin Layer Chromatography. Thin layer chromatography, or TLC, is a method for analyzing mixtures by separating the compounds in the mixture. TLC can be used to help determine the number ...
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...Thin layer chromatography or tlc is a method for analyzing mixtures by separating the compounds in mixture can be used to help determine number of components identity and purity compound observing appearance product disappearance reactant it also monitor progress reaction sensitive technique microgram g quantities analyzed takes little time an analysis about minutes consists three steps spotting development visualization photographs each step are shown on course website first sample dissolved volatile easily evaporated solvent produce very dilute solution using micro pipet transfer small amount this one end plate case powdered silica gel that has been coated onto plastic sheet quickly evaporates leaves behind spot material placing bottom into shallow pool which then travels up capillary action as moves over original competition set between spotted polar tries hold its place move along with outcome depends upon balance among polarities if enough will some distance from location differen...