Gram Stain Introduction The Gram stain is a differential staining procedure used to categorize bacteria as Gram-positive or Gram-negative based on the chemical and physical properties of their cell walls. The bacteria are differentiated through a series of staining and decolorization steps. Gram-positive cells will stain purple and Gram-negative cells will stain red to pink. Supplies and Reagents 1. Personal protective equipment 2. Slide rack 3. Timer 4. Absorbent paper, such as bibulous paper 5. Water (tap water or deionized) ...
Code: A1 Version: V1 National Public Health Laboratory Effective per: 1-8-2013 Retrieve per: 1-8-2015 Pages: 4 Signature authorizer: 1. Gram Staining 2. Objectives & Scope Gram staining technique is used for staining bacteria, yeasts and aerobic actinomycetes. This Standard Operating Procedure is applicable to all technical staff in the microbiology section that have been trained in performing this procedure and are competent and authorized to perform this procedure. 3. Abbreviations and definitions For general abbreviations, definitions and terms refer ...
Gram Stain Protocols | | Created: Friday, 30 September 2005 Author • Ann C. Smith • Marise A. Hussey Information History The Gram stain was first used in 1884 by Hans Christian Gram (Gram,1884). Gram was searching for a method that would allow visualization of cocci in tissue sections of lungs of those who had died of pneumonia. Already available was a staining method designed by Robert Koch for visualizing turbercle bacilli. Gram devised his method that used Crystal ...
Gram Stain Introduction The Gram stain is a differential staining procedure used to categorize bacteria as Gram positive or Gram negative based on the chemical and physical properties of their cell’s walls. The bacteria are differentiated through a series of staining and decolorization steps. Gram positive cells will stain purple and Gram-negative cells will stain red to pink. Supplies and Reagents 1. Personal protective equipment (PPE) 3. 2. Slide rack 1. 3. Timer 4. Absorbent paper, such as bibulous ...
Standard Operating Procedure Subject Gram Stain Index Number Lab-8683 Section Laboratory Subsection Regional/Affiliates Category Departmental Contact Sarah Stoner Last Revised 5/29/2019 References Required document for Laboratory Accreditation by the College of American Pathologists (CAP), Centers for Medicare and Medicaid Services (CMS) and/or COLA. Applicable To Employees of the Gundersen Health System clinical laboratories, Gundersen Tri-County Hospital laboratories, St. Joseph’s Health Services laboratories, Gundersen Boscobel Area Hospital laboratories, Gundersen Moundview Hospital laboratories, and Gundersen Palmer Lutheran Hospital and Clinics laboratories ...
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