gram stain protocols created friday 30 september 2005 author ann c smith marise a hussey information history the gram stain was first used in 1884 by hans christian gram gram ...
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...Gram stain protocols created friday september author ann c smith marise a hussey information history the was first used in by hans christian searching for method that would allow visualization of cocci tissue sections lungs those who had died pneumonia already available staining designed robert koch visualizing turbercle bacilli devised his crystal violet gentian as primary an iodine solution mordant followed treatment with ethanol decolorizer this procedure left nuclei eukaryotic cells samples unstained while found succumbed to were stained blue worked series bacteria associated disease such suppurative arthritis following scarlet fever he however typhoid easily decolorized after and when added we now know organisms s are positive include streptococcus pneumoniae pyogenes from patients negativebacteria salmonella typhi is purpose fundamental phenotypic characterization differentiates domain according cell wall structure have thick peptidoglycan layer purple negative thin red pink theo...