isolation of pure cultures a pure culture theoretically contains a single bacterial species there are a number of procedures available for the isolation of pure cultures from mixed populations a ...
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...Isolation of pure cultures a culture theoretically contains single bacterial species there are number procedures available for the from mixed populations may be isolated by use special media with specific chemical or physical agents that allow enrichment selection one organism over another differential and selective will utilized later in this course simpler methods include i spread plating on solid agar medium glass spreader ii streak loop purpose is to isolate individual cells colony forming units nutrient both require understanding aseptic technique asepsis can defined as absence infectious microorganisms however term usually applied any designed keep unwanted contaminating sterile materials first period material seven ml dilution tubes saline plates pipets aka hockey stick ethyl alcohol beaker warning away flame overnight broth staphylococcus aureus serratia marcescens procedure work pairs plate bacteria per unit volume sample reduced serial before surface an prepare dilutions show...