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...Pure culture practical techniques structure introduction an plating method agar shake tube most probable number mpn laser tweezers technology review questions exercise isolation of bacteria by performing streak plate focused on sub culturing we learnt the involved in i e process transfer from one medium to another or parent growth source what is role basically for maintaining and transferring various microbiological test procedures as a technique important you would realize that studies require microorganisms form how obtain these forms single type microorganism this focus introduces concept ways obtaining it after going through will be able understand importance careful study individual microbial species objectives studying conducting exercises given herewith explain describe methods employed cultures prepare maintain can isolated natural environments like soil food water sewage decomposing matter even infectious materials such pus sputum urine etc may recall about sources unit theory...