Protein Isolation Using Phenol

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File: Protein Isolation Using Phenol

icbr proteomics core protein extraction using phenol and methanolic ammonium acetate precipitation 1 put fresh tissue in 1 5 ml tube on ice with water 2 add 150 ul of ...

Filetype PDF | Posted on 15 Sep 2022 | 2 years ago

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...Icbr proteomics core protein extraction using phenol and methanolic ammonium acetate precipitation put fresh tissue in ml tube on ice with water add ul of media m tris hcl ph mm edta sucrose sonicate less than one minute cool down adjust the dial for optimization output power watt will be do not exceed over repeat step trice mercaptoethanol buffered mixing well vortexing centrifuge min at g c transfer phase should top there is into new back extract aqueous by combine first if your sample includes high salt reversed precipitate extracted proteins adding volumes cold methanol stored vortex incubate least h or overnight collect centrifugation i preferred this procedure wash pellet twice acetone resuspend final buffer pipetting room temperature agitation heat...

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Category: Uncategorized / Uncategorized
Posted on: 15 Sep 2022 | 2 years ago
Tag: download pdf, protein, isolation, using, phenol,
Filetype: PDF
File size: 0.24 MB
Views: 58x
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