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...Cryptogamie mycologie adac tous droits reserves an optimized protocol of single spore isolation for fungi ke zhang yuan ying su lei cai state key laboratory mycology institute microbiology chinese academy sciences beijing p r china abstract pure culture is important taxonomic physiological and various application studies many protocols obtaining cultures have been reported which was used most frequently in mycological however previously methods were relatively complicated time consuming this paper introduced compared to previous favorable terms its lower contamination rate less requires only simplified procedures with common easily accessible apparatus techniques phytopathogens strain introduction essential phyto pathology surface sterilization are the two strategies obtain hawksworth method simple convenient when plant materials infected by more than one fungal species fast growing often suppress others during addition endo phytes also isolated together targeted pathogenic example cap...