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...Ss leishman s stain solution is used for blood staining composition ingredients gm methanol absolute ml formula adjusted standardized to suit performance parameters directions freshly prepare and rapidly air dry film cover the with allow act minute in fixes preparation add double volume of distilled water slide mix diluted minutes wash or phosphate buffer ph drain examine principle interpretation microscopy smears it provides excellent quality generally differentiate identify leucocytes malaria parasites trypanosomas based on a methanolic mixture polychromed methylene blue i e demethylated into various azures eosin stock stable also serves purpose directly fixing smear eliminating prefixing step control appearance coloured clarity clear without any particles microscopic examination carried out characteristic observed under microscope by using oil immersion lens results nuclear basophilic cytoplasmic components neutrophilic granules lilac eosinophilic orange nucleoli violet red cells pi...