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...Blood culture collection procedure principle detection of organisms in a patient s has diagnostic and prognostic importance when bacteria multiply at rate that exceeds the capacity reticuloendothelial system to remove them bacteremia results enter stream from extravascular sites via lymphatic vessels cultures are essential diagnosis treatment etiologic agent sepsis bacterial constitutes one most serious infectious diseases therefore expeditious identification borne fungal pathogens is important functions microbiology laboratory guidelines achieve this end described materials bact alert aerobic bottle blue anaerobic purple adapter wampole isolator tube ml for filamentous acid fast bacilli afb pediatric chloraprep step frepp applicator medi flex inc store room temperature sepp butterfly needle alcohol pad safety during all routine operation maintenance procedures equipment protective required appropriate devices will be utilized ensure standard precautions compliance methods general cons...