animal tissue culture with special emphasis on mammalian cell culture by dr luna phukan contents 1 introduction 2 history of tissue culture 3 synopses of frequently used terms 4 concepts ...
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...Animal tissue culture with special emphasis on mammalian cell by dr luna phukan contents introduction history of synopses frequently used terms concepts in application is the growth tissues or cells an artificial medium separate from organism this typically facilitated via use a liquid semi solid such as broth agar th century english physiologist sydney ringer developed salt solutions containing chlorides sodium potassium calcium and magnesium suitable for maintaining beating isolated heart outside body wilhelm roux removed portion medullary plate embryonic chicken maintained it warm saline solution several days establishing principle ross granville harrison working at johns hopkins medical school then yale university published results his experiments to methodology techniques were advanced significantly s support research virology growing viruses cultures allowed preparation purified manufacture vaccines injectable polio vaccine jonas salk was one first products mass produced using ma...