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...Guidelines for hematoxylin eosin staining national society histotechnology northview drive suite bowie maryland phone fax email histo nsh org website www and the desired end result of a tissue stained with h e is based upon what seems to be almost infinite factors pathologists or diagnostician have individual preferences section thickness intensities shades choice which reagents use must take into consideration cost method manual vs automatic option purchasing commercially prepared technician safety administration policies convenience availability quality technical limitations as well personal preference realizing various options these are designed assist histotechnician in obtaining individually preferred results consistently i clearants xylene substitutes used two different stages deparaffinization removal paraffin troubleshooting if not totally removed from sections color intensity may decreased irregular spotty within recommendations three changes minutes each station an stainer wh...