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 128x       Filetype PDF       File size 0.10 MB       Source: chemistry.miamioh.edu


File: Column
How to run column chromatography Column chromatography is a commonly used purification technique in labs of organic chemistry. Done right it can simply and quickly isolate desired compounds from a ...

icon picture PDF Filetype PDF | Posted on 14 Sep 2022 | 2 years ago
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...How to run column chromatography is a commonly used purification technique in labs of organic chemistry done right it can simply and quickly isolate desired compounds from mixture but like many aspects practical the quick efficient setting up running something that take time master here are some instructions help you set introduction typical fig stationary phase solid adsorbent normally silica gel sio placed vertical glass mobile liquid added top flows down through by either gravity or external pressure flash separation achieved varying absorption on interaction between phases figure general preparing columns we use non fritted will need be plugged with wool cotton prevent loss out bottom positioning awkward at first left ball should large enough plug not so densely packed restricts solvent flow piece size tip your little finger suitable for most position securely narrowest part using long rod other device clamp close tap stopcock add layer sand until reaches main body approx cm this g...
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