Methods of Plant Tissue Culture: Plant tissue culture includes two major methods: (A) Type of in vitro growth-callus and suspension cultures. (B) Type of explant— single cell culture, shoot and root cultures, somatic embryo culture, meristem culture, anther culture and haploid production, protoplast culture and somatic hybridisation, embryo culture, ovule culture, ovary culture, etc. Types of Plant Tissue Culture: Callus and Suspension Cultures: In callus culture, cell division in explant forms a callus. Callus is irregular unorganised and undifferentiated mass of actively dividing cells. Darkness and solid medium gelled by agar stimulates callus formation. The medium ordinarily contains the auxin ...
Setting Up a Tissue Culture Lab Any laboratory, in which tissue culture techniques are performed, regardless of the specific purpose, must contain a number of basic facilities. These usually include the following: • A general washing area • A media preparation, sterilization, and storage area • An aseptic transfer area • Environmentally controlled incubators or culture rooms • An observation/data collection area. Nutrient media: The type and composition of culture media very strongly govern the growth and morphogenesis of plant tissues. The choice of tissue culture medium largely depends upon the species to be cultured. For e.g. some species ...
Objectives Of the course At the end of this course students should be able to: explain the principles and technical requirements of tissue Culture. Discuss applications of plant tissue culture. practice the most common methods of plant cell and tissue culture. propagate rare and endangered plant species by laboratory method. 2 Chapter 1. Introduction Plant biotechnology - a scientific technique that adapts plants for specific purposes by cross-breeding, extending their growing seasons, adjusting height, color and texture and several other mechanisms. What is biotechnology? Bio = Biology; Technology = Application= “The application of Biology” the methods and techniques that ...
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