Differences between Conventional and Fluorescent Microscope aThe Conventional a A fluorescence e microscope, uses a microscope uses much higher intensity visible light (400- light source which 700 nanometers) excites a fluorescent species in a sample of to illuminate and interest. This produce a fluorescent species in turn emits a lower magnified image energy light of a of a sample. longer wavelength that produces the magnified image Dr.T.V.Rao MDinstead of the original 2 light source. What is Fluorescence? e aFluorescence is light produced by a substance when it is stimulated by another light. Fluorescence is called " ...
Microscopes • Microscopes and microbiology linking advance •The microscope is the microbiologist’s most basic tool . • Microscopes use lenses to magnify object’s images. • There are two types of microscopes: Light microscopes (4 types) Electron microscope (2 types) Light microscope used to examine cells at relatively low magnifications and electron microscope used to lock at cells and cell structure at very high magnification. Some Principles of Light Microscopy o Compound light microscope uses visible light to illuminate cells o Many different types of light microscopy: o Bright-field o Phase-contrast o Dark-field o Fluorescence Some Principles of Light ...
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