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...Spoje v hplc color code peek tubing nevhodne spojeni rozsiovani elunich zon netsnost systemu velky tlakovy spad zvyseni sumu baseline kapilara sroub ferrulka tsnici krouek kriticka vzdalenost pro kade hardwarove zaizeni konec kapilary reversed phase chromatography mobile selection in rp generally consist of mixtures water and miscible organic solvent methanol acetonitrile ethanol decreasing polarity isopropanol increasing elution dimethylformamide power propan ol dioxane tetrahydrofuran non aqueous eluents are used for highly polar compounds the with have higher viscosity produced back pressure than pure solvents common acids bases low strong dipole available highest purity adjustment cheap is only one parameter to change separation another selectivity term not all could be possible chemical interaction miscibility issue toxic flammable volatile stationary phases c modified silica most providing high retention other phenyl cn diol nh lower alternative carbon load strenght estimated fro...