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...Introduction cell culture is the process by which prokaryotic eukaryotic or plant cells are grown under controlled conditions but in practice it refers to culturing of derived from animal was first successfully undertaken ross harrison roux for time maintained embryonic chick a major development s technology use antibiotics inhibits growth contaminants second trypsin remove adherent subculture further vessel third chemically defined medium why used areas where currently playing role model systems studying basic biology interactions between disease causing agents and effects drugs on triggering aging nutritional studies toxicity testing study new cancer research function various chemicals virus radiation convert normal cultured cancerous contd virology cultivation vaccine production also there infectious cycle genetic engineering commercial proteins large scale viruses e g polio rabies chicken pox hepatitis b measles gene therapy having functional can be replaced non tissue vitro organs...