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picture1_Differential Centrifugation Slideshare 77394 | 4   Rardin


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File: Differential Centrifugation Slideshare 77394 | 4 Rardin
sirt3 regulated mitochondrial lysine acetylation cytos ol om im s im matri x type 2 diabetes metabolic syndromes high low fat diet aging cancer pnas 2013 110 16 6601 6606 ...

icon picture PPTX Filetype Power Point PPTX | Posted on 03 Sep 2022 | 3 years ago
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...Sirt regulated mitochondrial lysine acetylation cytos ol om im s matri x type diabetes metabolic syndromes high low fat diet aging cancer pnas strategy for identification and quantitation of the acetylome in mouse liver mitochondria were purified from wt ko mice by differential centrifugation normalized to total protein perform a trypsin digest with two replicates per sample control process variability spike acetyl standard lvssvsdlp ack r as loading polyclonal antibody combination enrichment acetylated peptides injection ran on triple tof label free relative using ms filtering published spectral library information panorama skyline quantitative tool discovery proteomics experiments follow few peptide analytes samples n interface tools mass spectrometer platform manufacturer independent qqtof ft its search engine redundant ion chromatograms raw data peak integration mol cell elqhhvkacsvtapyk area acsm k replicate view m...

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