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...Liposomes lipid vessicles can self assemble to spheres at t g amphiphilic nature of the lipids phospholipids polar group and hydrophobic chain with core image from http www reemazeineldin com liposome html for drug delivery biocompatible both hydrophilic drugs be loaded journal cancer science therapy separation important separate unloaded molecules size molecular weight ultra centrifugation nm pellet down due higher sedimentation coefficient fusion is possible breakage loss molecule dialysis cut off range bag density gradient sucrose or ficoll combined ultracentrifugation column chromatography most effective method morphology maintained expensive large scale a problem statement develop novel simple technique rapid cost potential application...