NCCIH Mission Define, through rigorous scientific investigation, the usefulness and safety of complementary and integrative health interventions and their roles in improving health and health care Strategic Plan Objective 1: Advance Fundamental Science and Methods Development NCCIH and Syn Bio 2012: DP1 AT007886; Synthetic Biology Platforms for Natural Product Discovery and Biosynthesis (Smolke) NCCIH and Syn Bio 2015: DP2 AT009148; In Situ Natural Product Labeling and Applications (Zhang) NCCIH and Syn Bio 2019: R01 AT010593; Synthetic biology tools for scalable production of medicinal plant terpenes (Sattely) NCCIH and Syn Bio Methods Development in Natural Products Chemistry (SBIR/STTR) Synthetic Biology for ...
Site-directed mutagenesis is a molecular biology method that is used to make specific and intentional changes to the DNA sequence of a gene and any gene products. Also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, it is used for investigating the structure and biological activity of DNA, RNA, and protein molecules. Site-directed mutagenesis is one of the most important techniques in laboratory for introducing mutation into a DNA sequence. However, with decreasing costs of oligonucleotide synthesis, artificial gene synthesis is now occasionally used as an alternative to site-directed mutagenesis. Basic mechanism The basic procedure requires the synthesis of a ...
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