Site Directed Mutagenesis Slideshare 68430

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File: Site Directed Mutagenesis Slideshare 68430 | Lec No 5(5)

site directed mutagenesis is a molecular biology method that is used to make specific and intentional changes to the dna sequence of a gene and any gene products also called ...

Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago

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...Site directed mutagenesis is a molecular biology method that used to make specific and intentional changes the dna sequence of gene any products also called or oligonucleotide it for investigating structure biological activity rna protein molecules one most important techniques in laboratory introducing mutation into however with decreasing costs synthesis artificial now occasionally as an alternative basic mechanism procedure requires short primer this synthetic contains desired complementary template around so can hybridize interest may be single base change point multiple deletion insertion strand then extended using polymerase which copies rest thus copied mutated introduced host cell vector cloned finally mutants are selected by sequencing check they contain approaches kunkel s fragment inserted phagemid such mmp transformed e coli strain deficient two enzymes dutpase dut uracil deglycosidase ung both part repair pathway deficiency prevents breakdown dutp resulting high level remo...

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Posted on: 28 Aug 2022 | 2 years ago
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