1 fluorescein 2 rhodamine 1 chemical coupling of a reporter molecule to a nucleotide precursor 2 after incorporation into dna the reporter groups can be specifically bound by an affinity ...
Filetype Power Point PPTX | Posted on 30 Aug 2022 | 3 years ago
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...Fluorescein rhodamine chemical coupling of a reporter molecule to nucleotide precursor after incorporation into dna the groups can be specifically bound by an affinity very high conjugated marker or group which detected in suitable assay molecules on modified need protrude sufficiently far from nucleic acid backbone so bind efficiently spacer carbon atoms is needed separate anti digoxigenin antibodies coupled either alkaline phosphatase ap horseradish peroxidase hrp for colorimetric and chemiluminescent fluorescent detection cdp star substrate biotin streptavidin system streptomyces avidinii plant steroid obtained digitalis species specific antibody both are linked uracil t has replaced with dutp hybridization probes recognized during incubation nbt bcip this enzyme deposits brownish purple precipitate embryos then turns deep blue color exposure ethanol way cells have produced particular mrna stained pattern across entire embryo seen such as simple seven stripe ftz gene shown here...