Sterilization & Disinfection Sterilization & Disinfection Sterilization: Is the killing or removal of all micro-organisims, including bacterial spores, which are highly resistant. e.g.: - o Sterilization by autoclave (121 c , 15psi). - Ethyl oxide gas for surgical instruments. - Filtration for intravenous solution. Classification of sterilization agents: 1- Physical agents: a- heat b- filtration c- radiation 2- Chemical agents: Classification of Sterilization Methods Classification of Sterilization Methods Physical Sterilization Chemical Sterilization Radiation Filtration Heat Direct Sun Light Non Ionizing Radiation Moist Heat Dry Heat Ionizing Radiation Infrared Radiation Flaming Sterilization at a temp. Below 100Co o ...
Definition of Frequently Used Terms • Sterilization –Removes all viable microorganisms including viruses –Material is said to be sterile –Usually for inanimate objects –Mostly performed with heat Autoclave use saturated steam under pressure to reach temperatures (above boiling) 2 Definitions. • Disinfection –disinfectants • agents, usually chemical, used for disinfection • 5% bleach solution • The use of a physical process or chemical agent (disinfectant) to destroy vegetative pathogens • Does not destroy bacterial endospores • Usually used only on inanimate objects • Also removes toxins Decontamination & Sanitization • Any cleansing technique that ...
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