ENZYME-LINKED IMMUNO SORBENT ASSAY (ELISA is a test that uses antibodies and color change to identify a substance. ELISA is a popular format of a "wet-lab" type analytic biochemistry assay that uses a solid-phase enzyme immunoassay (EIA) to detect the presence of a substance, usually an antigen, in a liquid sample or wet sample. The ELISA has been used as a diagnostic tool in medicine and plant pathology, as well as a quality-control check in various industries ...
INTRODUCTION • Hormonal assay is the test carried out on sample of blood to detect and measure the level of a hormone. • It is performed to confirm hormonal abnormalities and also during treatment and after treatment of hormonal abnormalities. Measurement of Hormone Concentration in the Blood • Hormones are present in the blood in extremely minute quantities. • Some concentrations are as low as one billionth of a milligram (1 picogram) per milliliter. Therefore, it was very difficult to ...
• Microparticle Enzyme Immunoassay (MEIA) is an immunoassay method that utilizes the isolation of antibody/antigen complexes on a solid phase surface of small beads called microparticles. • MEIA has been widely adapted to automate the measurement of large molecules such as markers associated with cardiac, fertility, cancer, metabolic, hepatitis, and thyroid testing. • Chemiluminecent microparticle immunoassay CMIA used for quantitative determination of human thyroid stimulating hormone in serum and plasma. • In ARCHITECT instrument, TSH assay is a two-step immunoassay ...
ELISA(enzyme-linked immunosorbent assay) is an antigen antibody reaction. In 1971, ELISA was introduced by Peter Perlmann and Eva Engvall at Stockholm University in Sweden. It is a common laboratory technique which is usually used to measure the concentration of antibodies or antigens in blood. ELISA is a plate based assay technique which is used for detecting and quantifying substances such as peptides, proteins, antibodies and hormones. An enzyme conjugated with an antibody reacts with colorless substrate to generate a colored ...
Topics: Laboratory methods of assessment of humoral immunity. Radioimmunoassay, enzyme immunoassay Laboratory measurement of specific IgE antibodies. Laboratory measurement of autoantibodies. Laboratory methods of assessment of cellular immunity. Flow cytometry - principles, practical use. Laboratory methods of assessment of humoral immunity all methods use an antigen (Ag) – antibody(Ab) reaction as their primary means of detection we assess either Ag or Ab Laboratory methods of assessment of humoral immunity • Precipitation – immunodiffussion immunoelectrophoresis turbidimetry nephelometry • Agglutination • Radioimmunoassay ...
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