elisa enzyme linked immunosorbent assay is an antigen antibody reaction in 1971 elisa was introduced by peter perlmann and eva engvall at stockholm university in sweden it is a common ...
Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago
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...Elisa enzyme linked immunosorbent assay is an antigen antibody reaction in was introduced by peter perlmann and eva engvall at stockholm university sweden it a common laboratory technique which usually used to measure the concentration of antibodies or antigens blood plate based for detecting quantifying substances such as peptides proteins hormones conjugated with reacts colorless substrate generate colored product called chromogenic number enzymes have been alkaline phosphatase horse radish peroxidase beta galactosidase specific ortho phenyldiamine dihydrochloride paranitrophenyl phosphate are hydrolysed above give end types frequently there on basis binding structure between direct indirect sandwich competitive intended detection quantification analyte e g etc from within complex biological sample four different formats simplest quickest perform but some disadvantages associated this method can be detected quantitatively determined coated microtiter well serum other containing prima...