Overview Objectives Background information Materials Procedures: Part 1: Restriction of Lambda DNA Part 2: Gel Electrophoresis of Lambda DNA Part 3 : DNA Extraction Part 4: Chemical Tests Closing and Feedback Objective Restrict Lambda DNA and analyze by Gel Electrophoresis Extract a sizable amount of DNA Understand and use basic biochemical techniques: DNA isolation method Gel electrophoresis Biochemical tests Understand role of ingredients used in experiment Plant Cell Structure Plant Cell Structure: The Nucleus Nuclear envelope Double layer of lipids Protect DNA from DNAses Chromatin Made of DNA and protein chromatin DNA cell nucleus chromosome DNA Properties Structure Helical backbone ...
Instructors - Bio-Rad Curriculum and Training Specialists Sherri Andrews, Ph.D., Eastern US sherri_andrews@bio-rad.com Damon Tighe, Western US damon_tighe@bio-rad.com Leigh Brown, M.A., Central US leigh_brown@bio-rad.com 2 Biotechnology Explorer™ | explorer.bio-rad.com Workshop Timeline Introduction Background on DNA Extraction Extract Genomic DNA from Cheek Cells Prepare DNA Necklaces 3 Biotechnology Explorer™ | explorer.bio-rad.com Relevance of DNA Isolation Isolation of DNA is often the first step before further analysis – DNA profiling – Cloning – Disease diagnosis – DNA sequencing – Genetically modified organisms (GMO) -agriculture, pharmaceutical – Environmental testing, biodefense 4 Biotechnology Explorer™ | ...
Introduction The isolation of nucleic acids derived from archived clinical material can provide a powerful tool in studies of diseased tissue at both the genomic and gene expression level. Standard preservation techniques for storage of biological tissue samples involve formalin- fixation and paraffin-embedding (FFPE). While the high reactivity of these chemicals makes them ideal for maintaining tissue structure, isolating genomic material for molecular analysis can be challenging due to: (1) trapping and modification of nucleic acids in extensive protein- protein and protein-nucleic acid crosslinks, (2) fragmentation and chemical modification of the RNA or DNA that occurs during the FFPE process ...
Identification of molecular biology : It is the science deals with macromolecules and to understand the five basic behaviors patterns(growth , division , specialization ,movement and interaction)in terms of the fine molecules responsible for them including (DNA ,RNA and Protein) • Freidrich Miescher (1869) : was a Swiss physician and biologist. He was the first researcher who isolate and identify nucleic acid. It was a phosphate-rich chemicals, which he called nuclein (now nucleic acids), from the nuclei of white blood cells without knowing it responsibility about inheritance. so he knew much of the nucleic acid chemistry but their function remained unknown till ...
PPT - Power Point | 1.69 MB | Free Download
