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picture1_Protein Purification Slideshare 68346 | Gpsr Study Ffpe Isolation 5


picture2_Protein Purification Slideshare 68346 | Gpsr Study Ffpe Isolation 5 picture3_Protein Purification Slideshare 68346 | Gpsr Study Ffpe Isolation 5

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File: Protein Purification Slideshare 68346 | Gpsr Study Ffpe Isolation 5
Introduction The isolation of nucleic acids derived from archived clinical material can provide a powerful tool in studies of diseased tissue at both the genomic and gene expression level. Standard ...

icon picture PPTX Filetype Power Point PPTX | Posted on 28 Aug 2022 | 2 years ago
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...Introduction the isolation of nucleic acids derived from archived clinical material can provide a powerful tool in studies diseased tissue at both genomic and gene expression level standard preservation techniques for storage biological samples involve formalin fixation paraffin embedding ffpe while high reactivity these chemicals makes them ideal maintaining structure isolating molecular analysis be challenging due to trapping modification extensive protein acid crosslinks fragmentation chemical rna or dna that occurs during process reagents used blocks slide preparations complicate purification inhibit downstream enzymatic reactions effects this long times common with banked state is incompatible many improved protocols designed specifically meet challenges working are commercially available study we examined two commonly methods goals measuring mass purity integrity isolated determining if automating one results similar amount quality as manual method ohsu integrated genomics labora...
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