manipulating nucleic acids to understand the basic techniques used to work with nucleic acids remember that nucleic acids are macromolecules made of nucleotides a sugar a phosphate and a nitrogenous ...
Filetype Power Point PPTX | Posted on 31 Aug 2022 | 4 years ago
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...Manipulating nucleic acids to understand the basic techniques used work with remember that are macromolecules made of nucleotides a sugar phosphate and nitrogenous base linked by phosphodiester bonds groups on these molecules each have net negative charge understanding processes dna replication transcription can enable manipulation in laboratory setting extraction or rna must first be isolated extracted from cells various extract different types most acid involve steps break open cell use enzymatic reactions destroy all not desired inactivated using enzymes such as proteases down proteins ribonucleases rnases gel electrophoresis mobilized an electric field is technique separate basis size this matrix observed fluorescent colored dyes distinct fragments appear bands at specific distances top electrode end their amplification polymerase chain reaction pcr amplify regions for further analysis many purposes laboratories cloning gene analyze genetic diseases identification contaminant forei...